Research.

I employ biological, computational, and mathematical approaches to life science problem solving.

Curriculum Vitae

Publications

Original Research | Structural Biology

Structural basis for autophagy inhibition by the human Rubicon-Rab7 complex.

2020 | Proceedings of the National Academy of Science | Bhargava H.K., Tabata K., Byck J.M., Hamasaki M., Farrell D.P., Anishchenko I., DiMaio F., Im Y.J., Yoshimori T., Hurley J.H.

We report the novel atomic structure of autophagy inhibitor Rubicon bound to GTPase Rab7. Our work provides a roadmap to block Rubicon localization and activity in order to upregulate autophagy, a major drug development goal.

PDF Journal Website PDB Entry

Original Research | AI Driven Medicine

Computationally derived image signature of stromal morphology is prognostic of prostate cancer recurrence following prostatectomy in African American patients.

2020 | Clinical Cancer Research | Bhargava H.K., Leo, P., Elliott, R., Janowcyzk, A., Whitney, J., Gupta, S., Fu, P., Yamoah, K., Rebbeck, T., Feldman, D., Lal, P., Madabhushi, A.

We developed computer vision and machine learning to predict prostate cancer recurrence risk based on quantitative measurements of the intratumoral stroma computed from digitized H&E images.

PDF Journal Website

Original Research | Protein Engineering, Chemical Biology

The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.

2021 | Nature Chemical Biology | Deo, C.*, Abdelfattah, A.S.*, Bhargava, H.K., Berro, A., Falco, N., Moeyaert, B., Chupanova, M., Lavis, L.D., Schreiter, E.R.

We introduce a new platform for ‘chemigenetic’ fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. This approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in neurons.

PDF Journal Website

Original Research | Clinical Pathology

PD-L1 Expression Reveals Significant Association with Squamous Differentiation in Upper Tract Urothelial Carcinoma.

2019 | American Journal of Clinical Pathology | Arriola, A., Farahani, S., Bhargava, H.K., Guzzo, T., Brooks, J., Lal, P.

This study comprehensively reviews programmed death 1 receptor (PD-1)–positive and CD8+ tumor-infiltrating lymphocytes (TILs) and programmed death ligand 1 (PD-L1) expression on tumor epithelium (TE).

PDF Journal Website